Plasmid

Part:BBa_J23076

Designed by: John Anderson   Group: iGEM06_Berkeley   (2006-09-06)


pSB3C6-like RFP reporter

This is a p15A/CmR plasmid designed for shortcuts to promoter reporters with RFP, or as a shortcut to pSB3C6 variants with the r0040 Ptet promoter part. The EcoRI/PstI region goes:

E-X-[Ptet]-S-[stRBS][RFP][TT]-P

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 78
    Illegal PstI site found at 961
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 1808
    Illegal SpeI site found at 78
    Illegal PstI site found at 961
    Illegal NotI site found at 7
    Illegal NotI site found at 954
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 78
    Illegal PstI site found at 961
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 78
    Illegal PstI site found at 961
    Illegal AgeI site found at 657
    Illegal AgeI site found at 769
    Illegal AgeI site found at 1398
    Illegal AgeI site found at 1722
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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